The latest available version of QuantCenter is 2.3. Please get in touch with us if you are interested! 

QuantCenter is intended for use in tissue classification, IHC quantification and molecular pathology. It consists of ten individual modules that can be freely combined for world-class research results:

ScriptQuant is a brand-new image analysis module for QuantCenter.  It provides a shell for user-created image analysis scripts in Python for maximum flexibility for the research user, enabling them to run their own image analysis algorithms within the QuantCenter framework.

QuantCenter provides a range of image analysis modules that can be freely combined for best results.

Color- and pattern-based image analysis solutions are used to identify different tissue elements in stained tissue. Instead of the time-consuming manual annotation, automated detection and annotation of a region of interest is available with a quick training on relevant tissue samples.

HistoQuant is an image segmentation module which identifies stained tissue elements based on color and intensity features. This is an adequate solution for double-stain quantification or multiplex fluorescent analysis, with dual stain colocalization option. This module can analyze fluorescent and brightfield slides.

PatternQuant is a trainable pattern recognition module for tissue classification, tissue pre-segmentation and identification of several tissue structures. The artificial intelligence-based algorithm is able to learn and classify various tissue types based on their texture patterns and colour features. This module can analyze fluorescent and brightfield slides.

PatternQuant Plus is an improved alternative to PatternQuant for more challenging samples (low visual difference between the staining of tissue types within the sample) and for complex tissue segmentation projects when thorough training using deep learning on multiple slides is needed. This module can analyze fluorescent and brightfield slides.

MembraneQuant is a cell membrane detection software application that can be used for the quantification of IHC stained histological samples. This module measures cell morphology and stain density, reports intensity-based core ranges, overall scores and positivity percentages (including H-Score), distinguishing the continuous membrane stain from the moderate stain.

The Swiss army knife of 3DHISTECH’s image analysis tools, CellQuant is a versatile cell detection application, an optimal solution for investigating various IHC stainings (best suited for Ki67 slides). The application is adequate for cell nuclei, cytoplasmic and membrane marker quantification. The software reports the results like positivity ranges of cell nuclei, cytoplasm or membrane signals. This module can analyze fluorescent and brightfield slides.

DensitoQuant is an easy-to-use, fast and accurate, stain-intensity-based IHC quantification tool. The application identifies the positive stain, based on an automatic color separation method through which individual positive pixels are counted and classified based on intensity and threshold ranges. This way, an H-Score can be calculated based on the proportion of positive and negative pixels.

Fluorescent in situ hybridization (FISH) and Chromogenic in situ hybridization (CISH) are routinely used methods in histopathological diagnostics. The visual evaluation of these samples is a difficult task, and typically a time-consuming process and prone to human errors that can be eliminated by using image analysis solutions.

FISHQuant is a powerful cancer and cytogenetic application dedicated to quantifying  FISH  (Fluorescent In  Situ  Hybridization) signals on tissue samples of solid tumour diseases like breast cancer, lung cancer, sarcoma symptoms, and lymphomas. This module is suitable for examining genetic mutations (gene amplification, deletion) and chromosomal aberrations (polysomy, aneusomy) on FISH slides. This module has IVD approval.

CISHQuant quantifies CISH (Chromogenic In Situ Hybridization) stained samples. The algorithm can be calibrated to the staining protocol and quality by using an integrated colour setting tool. This module is suitable for examining genetic mutations (gene amplification, deletion) and chromosomal aberrations (polysomy, aneusomy) on CISH slides.

Using the batch analysis mode, multiple digital slides can be examined in the background, saving a significant amount of time. Measurements can be run after slides, annotations and profiles have been selected. All measurement results can be exported into an Excel file.

An integrated data visualization mode is available for each module. The result can be visualized on a  table,  scatterplot, pie chart or a histogram by using this feature. There are integrated galleries where the detected and classified cells can be presented.

QuantCenter also offers an integrated data visualization mode and a batch analysis mode. 

Her2stain quantification using PatternQuant and MembraneQuant

Breast Cancer Tissue Analysis using PatternQuant and NuclearQuant